Intracellular signalling – VASP phosphorylation
VASP (vasodilator stimulated phosphoprotein) phosphorylation is routinely measured as a surrogate measure for cAMP levels. Measurement of VASP-P enables the monitoring of agents that act at cell-surface receptors linked to stimulation or inhibition of adenylate cyclase and agents that regulate the activity of phosphodiesterases.
Especially developed by the team, our novel technology VASPFix provides a sensitive assay for monitoring VASP phosphorylation in platelets. We offer an assay that is very simple to perform, with the added advantage that, once treated with VASPFix, the samples can be frozen and analysed by flow cytometry at any point, making this service time-efficient and flexible.
P2Y12 and EP3 antagonists promote the inhibitory effects of natural modulators of platelet aggregation that act via cAMP. Platelets, 22(7): 504-515. DOI: 10.3109/09537104.2011.576284
‘VASPFix’ for measurement of VASP phosphorylation in platelets and for monitoring effects of P2Y12 antagonists. Thromb Haemost, 111(3): 539-548.
Comparison of the Biocytex and the VASPFix assays of VASP phosphorylation in coronary-artery disease patients treated with clopidogrel plus aspirin.
http://onlinelibrary.wiley.com/doi/10.1111/jth.12993/epdf – page784