Platelet activation markers
Following activation platelets release the content of their granules and several markers become expressed on their membrane, which can be assessed by flow cytometry. P-selectin (CD62p) is a well-established, commonly used marker for measuring platelet activation; our scientists have extensive experience assessing platelet function using this approach.
Samples are fixed after stimulation and the analysis of P-selectin expression can be performed at a later stage. This is an end-point assay and a very small volume of whole blood is required to run this test, which makes this approach a desirable high-throughput platelet assay if multiple compounds/conditions need to be assessed.
A platelet P-selectin test predicts adverse cardiovascular events in patients with acute coronary syndromes treated with aspirin and clopidogrel. Platelets, 25(8), pp.612-618.
Clinical utility of remote platelet function measurement using P-selectin: assessment of aspirin, clopidogrel, and prasugrel and bleeding disorders. Platelets, 29(5): 425-430.
How does measurement of platelet P-selectin compare with other methods of measuring platelet function as a means of determining the effectiveness of antiplatelet therapy? Platelets, 30(3): 290-295.